Vps13a^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Vps13a^{tm1d(EUCOMM)Wtsi}
• Name: vacuolar protein sorting 13A; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:8189899
• Gene: Vps13a Location: Chr19:16592730-16758297 bp, - strand Genetic Position: Chr19, 11.71 cM
• Alliance: Vps13a^{tm1d(EUCOMM)Wtsi} page
• IMPC: Vps13a gene page

Mutation origin

• Mutant Cell Line: EPD0645_3_G01
• Germline Transmission: Earliest citation of germline transmission: J:373104
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 16731375 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 16732214. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:373104)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vps13a Mutation: 135 strains or lines available

References

• Original: J:373104 Xu P, et al., Impaired hematopoiesis and embryonic lethality at midgestation of mice lacking both lipid transfer proteins VPS13A and VPS13C. PLoS Biol. 2025 Sep;23(9):e3003393
• All: 1 reference(s)