Krt77em1Vbm
Endonuclease-mediated Allele Detail
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| Symbol: |
Krt77em1Vbm |
| Name: |
keratin 77; endonuclease-mediated mutation 1, Maik Dahlhoff |
| MGI ID: |
MGI:8187527 |
| Gene: |
Krt77 Location: Chr15:101767166-101778140 bp, - strand Genetic Position: Chr15, 57.08 cM
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| Alliance: |
Krt77em1Vbm page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:363249
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| Parent Cell Line: |
C2 (Nagy) (ES Cell)
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| Strain of Origin: |
C57BL/6NTac
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| Allele Type: |
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Endonuclease-mediated (Null/knockout) |
| Mutation: |
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Insertion
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Mutation details: CRISPR/cas9 mediated recombination using a guide RNA (5-CGGTCCTTCTTATCCTCCCGG-3) inserted a 7 bp sequence (GAATTCC) containing an EcoRI restriction site into exon 1 creating a premature stop codon. RT-PCR analysis of back skin confirmed the loss of expression in homozygous mice. Expression of KRT1 is elevated in the suprabasal layer of the epidermis in homozygous mice.
(J:363249)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Krt77 Mutation: |
31 strains or lines available
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| Original: |
J:363249 Ghorbanalipoor S, et al., The loss of keratin 77 in murine skin is functionally compensated by keratin 1. Biochim Biophys Acta Mol Cell Res. 2025 Feb;1872(2):119881 |
| All: |
1 reference(s) |
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Analysis Tools
