About   Help   FAQ
Krt77em1Vbm
Endonuclease-mediated Allele Detail
Summary
Symbol: Krt77em1Vbm
Name: keratin 77; endonuclease-mediated mutation 1, Maik Dahlhoff
MGI ID: MGI:8187527
Gene: Krt77  Location: Chr15:101767166-101778140 bp, - strand  Genetic Position: Chr15, 57.08 cM
Alliance: Krt77em1Vbm page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:363249
Parent Cell Line:  C2 (Nagy) (ES Cell)
Strain of Origin:  C57BL/6NTac
Mutation
description
Allele Type:    Endonuclease-mediated (Null/knockout)
Mutation:    Insertion
 
Mutation detailsCRISPR/cas9 mediated recombination using a guide RNA (5-CGGTCCTTCTTATCCTCCCGG-3) inserted a 7 bp sequence (GAATTCC) containing an EcoRI restriction site into exon 1 creating a premature stop codon. RT-PCR analysis of back skin confirmed the loss of expression in homozygous mice. Expression of KRT1 is elevated in the suprabasal layer of the epidermis in homozygous mice. (J:363249)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Krt77 Mutation:  31 strains or lines available
References
Original:  J:363249 Ghorbanalipoor S, et al., The loss of keratin 77 in murine skin is functionally compensated by keratin 1. Biochim Biophys Acta Mol Cell Res. 2025 Feb;1872(2):119881
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/18/2025
MGI 6.24
The Jackson Laboratory