Gt(ROSA)26Sor^{tm1(CAG-Atf4/EGFP,-tdTomato)Calak}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Atf4/EGFP,-tdTomato)Calak}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Nicole Calakos
• MGI ID: MGI:8174710
• Synonyms: Rosa26-DIO-SPOTlight
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Atf4/EGFP,-tdTomato)Calak} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The allele was generated by inserting a DIO-SPOTlight construct into the GT(ROSA)26Sor locus (Ai9 Addgene, 22799), replacing the Lox-STOP-Lox-tdTomato cassette. The final targeting construct consists of a CAG (CMV enhancer/chicken beta-actin) promoter, inward facing lox2272 and loxP sites upstream of the activating transcription factor 4 (Atf4) open reading frame 1 (uORF1), an inverted SPOTlight cassette (enhanced green fluorescent (EGFP) cDNA sequence inserted after the upstream open reading frame 2 (uORF2) methionine start codon of the Atf4 gene and a red fluorescent protein (tdTomato) cDNA replacing the Atf4 start codon), followed by a second lox2272 site and second loxP site in opposite orientations to the first, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), a BGH polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1096 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)