Cdkn2a^tm3(dre)Bzsh
Targeted Allele Detail

Summary

• Symbol: Cdkn2a^tm3(dre)Bzsh
• Name: cyclin dependent kinase inhibitor 2A; targeted mutation 3, Bin Zhou
• MGI ID: MGI:8172891
• Gene: Cdkn2a Location: Chr4:89192710-89212856 bp, - strand Genetic Position: Chr4, 42.15 cM, cytoband C3-C6
• Alliance: Cdkn2a^tm3(dre)Bzsh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:364659
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6 x 129S)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Recombinase)
• Mutation: Insertion
• Cdkn2a^tm3(dre)Bzsh expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cdkn2a (MGI:104738)

• Mutation details: A targeting vector containing the triple SV40 polyadenylation (tpA) that functions as transcription terminators (stop) flanked by two loxP sites (loxP-stop-loxP or LSL), self-cleaving peptide 2A sequence and a complementary DNA (cDNA) encoding Dre recombinase followed by a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), polyA signal, and FRT-flanked neomycin resistance cassette was inserted into the last exon of the Cdkn2a gene replacing the stop codon. (J:101977, J:364659)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cdkn2a (mouse) Summary of all recombinase alleles driven by Cdkn2a. Alliance mouse Cdkn2a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdkn2a Mutation: 59 strains or lines available

References

• Original: J:364659 Zhao H, et al., Identifying specific functional roles for senescence across cell types. Cell. 2024 Oct 1
• All: 2 reference(s)