Cby3^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cby3^{tm1b(EUCOMM)Hmgu}
• Name: chibby family member 3; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:8172321
• Synonyms: Cby3^-, Cby3 KO
• Gene: Cby3 Location: Chr11:50248588-50250526 bp, + strand Genetic Position: Chr11, 30.54 cM, cytoband B1.2
• Alliance: Cby3^{tm1b(EUCOMM)Hmgu} page
• IMPC: Cby3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:344681
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 50249413 of Chromosome 11 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 50250763. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon. Western blot analysis confirmed the complete absence of protein expression in testis lysates from adult homozygous mutant males. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:344681)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cby3 Mutation: 9 strains or lines available

References

• Original: J:344681 Hoque M, et al., The Cby3/ciBAR1 complex positions the annulus along the sperm flagellum during spermiogenesis. J Cell Biol. 2024 Mar 4;223(3)
• All: 1 reference(s)