Gt(ROSA)26Sor^{tm11(CAG-cre/ERT2,-flpo,-tdTomato*)Ben}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm11(CAG-cre/ERT2,-flpo,-tdTomato*)Ben}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 11, Rui Benedito
• MGI ID: MGI:8161960
• Synonyms: iSuRe-HadCre
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm11(CAG-cre/ERT2,-flpo,-tdTomato*)Ben} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:352091
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm11(CAG-cre/ERT2,-flpo,-tdTomato*)Ben} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The targeting construct inserted into the locus a CAG promoter, FRT site, loxP site, triple stop codons, loxP site, cre recombinase gene,loxN site, modified estrogen receptor (ERT2), loxN site, in-frame Thosea asigna virus 2A cleavage site (T2A; mediates ribosomal skipping), flpo recombinase gene, FRT site, and the Mb-Tomato gene (non-oligomerizing tandem-dimer DsRed fluorescent protein variant tdTomato with N-terminal 41 amino acid membrane-targeting tag sequence). (J:352091)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:352091 Garcia-Gonzalez I, et al., iSuRe-HadCre is an essential tool for effective conditional genetics. Nucleic Acids Res. 2024 Jul 22;52(13):e56
• All: 2 reference(s)