Tcf4^em1Mbu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tcf4^em1Mbu
• Name: transcription factor 4; endonuclease-mediated mutation 1, Meinrad Busslinger
• MGI ID: MGI:7869404
• Synonyms: Tcf4^AID
• Gene: Tcf4 Location: Chr18:69476500-69821038 bp, + strand Genetic Position: Chr18, 43.23 cM, cytoband E2
• Alliance: Tcf4^em1Mbu page

Mutation origin

• Strain of Origin: (C57BL/6J x CBA/J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Inducible degradation)
• Mutation: Insertion
• Mutation details:

  Two guide RNAs (5'-CAGATGTGAAAAGGTACGCT-3'; and 5'-TTAGGGTAATACAGCTGTTA -3') were used to insert a glycine linker, a minimal auxin-inducible degron sequence and the V5 epitope in frame to the last amino acid sequence of the transcription factor 4 (Tcf4) gene on chromosome 18. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tcf4 Mutation: 66 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)