Prdm1^em2Mbu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prdm1^em2Mbu
• Name: PR domain containing 1, with ZNF domain; endonuclease-mediated mutation 2, Meinrad Busslinger
• MGI ID: MGI:7869398
• Synonyms: Prdm1^AID
• Gene: Prdm1 Location: Chr10:44313173-44404497 bp, - strand Genetic Position: Chr10, 23.24 cM
• Alliance: Prdm1^em2Mbu page

Mutation origin

• Strain of Origin: (C57BL/6J x CBA/J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Inducible degradation)
• Mutation: Insertion
• Mutation details:

  A single guide RNA (5'-TGAAAATCTTAAGGATCCAT-3') was used to insert a glycine linker, a minimal auxin-inducible degron sequence and the V5 epitope in frame to the last amino acid sequence of the PR domain containing 1, with ZNF domain (Prdm1) gene on chromosome 10. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prdm1 Mutation: 70 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)