Tcf3^em1Mbu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tcf3^em1Mbu
• Name: transcription factor 3; endonuclease-mediated mutation 1, Meinrad Busslinger
• MGI ID: MGI:7869367
• Synonyms: Tcf3^AID
• Gene: Tcf3 Location: Chr10:80245348-80269481 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Tcf3^em1Mbu page

Mutation origin

• Strain of Origin: (C57BL/6J x CBA/J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Inducible degradation)
• Mutation: Insertion
• Mutation details:

  A single guide RNA (5'-AAGAAGCTGTGACGGCTCAC-3') was used to insert a glycine linker, a minimal auxin-inducible degron sequence and the V5 epitope in frame to the last amino acid sequence of the transcription factor 3 (Tcf3) gene on chromosome 10. (J:101977, J:353472)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tcf3 Mutation: 48 strains or lines available

References

• Original: J:353472 Fedl AS, et al., Transcriptional function of E2A, Ebf1, Pax5, Ikaros and Aiolos analyzed by in vivo acute protein degradation in early B cell development. Nat Immunol. 2024 Sep;25(9):1663-1677
• All: 2 reference(s)