Ebf1^em1Mbu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ebf1^em1Mbu
• Name: early B cell factor 1; endonuclease-mediated mutation 1, Meinrad Busslinger
• MGI ID: MGI:7869365
• Synonyms: Ebf1^AID
• Gene: Ebf1 Location: Chr11:44508144-44898918 bp, + strand Genetic Position: Chr11, 26.45 cM
• Alliance: Ebf1^em1Mbu page

Mutation origin

• Strain of Origin: (C57BL/6J x CBA/J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Inducible degradation)
• Mutation: Insertion
• Mutation details:

  A single guide RNA (5'-CGTCTTTGGTCGCAGCGGGG-3') was used to insert a glycine linker, a minimal auxin-inducible degron sequence and the V5 epitope in the 5'-part of exon 2 of the early B cell factor 1 (Ebf1) gene on chromosome 11. (J:101977, J:353472)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ebf1 Mutation: 75 strains or lines available

References

• Original: J:353472 Fedl AS, et al., Transcriptional function of E2A, Ebf1, Pax5, Ikaros and Aiolos analyzed by in vivo acute protein degradation in early B cell development. Nat Immunol. 2024 Sep;25(9):1663-1677
• All: 2 reference(s)