Cd1d2^em1Kblg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cd1d2^em1Kblg
• Name: CD1d2 antigen; endonuclease-mediated mutation 1, Kamel Benlagha
• MGI ID: MGI:7861921
• Gene: Cd1d2 Location: Chr3:86893875-86896843 bp, + strand Genetic Position: Chr3, 37.88 cM
• Alliance: Cd1d2^em1Kblg page

Mutation origin

• Strain of Origin: BALB/cAnNRj

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR-targeting of exon 1 deleted a T at position 23, inducing a downstream STOP codon. Thus, from the start codon ATG to the new STOP, a 38-aa protein was produced. CRISPR technology details: Cas9 mRNA and sgRNAs targeting exon1 were mixed and electroporated (NEPA21) into the pronuclei of the one-cell embryos. SgRNA2 5-CCTACCGTGCCTGTTGCTGT-3 and SgRNA4 5-CAGCAACAGGCAC GGTAGGT-3. (J:344733)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd1d2 Mutation: 26 strains or lines available

References

• Original: J:344733 Amable L, et al., Intrinsic factors and CD1d1 but not CD1d2 expression levels control invariant natural killer T cell subset differentiation. Nat Commun. 2023 Dec 1;14(1):7922
• All: 1 reference(s)