Ptprc^em1Jmar
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ptprc^em1Jmar
• Name: protein tyrosine phosphatase receptor type C; endonuclease-mediated mutation 1, Jacqueline Marvel
• MGI ID: MGI:7861639
• Synonyms: Ptprc^{em(K302E)Jmar}
• Gene: Ptprc Location: Chr1:137990599-138103446 bp, - strand Genetic Position: Chr1, 60.73 cM
• Alliance: Ptprc^em1Jmar page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Single point mutation
• Mutation details: CRISPR genome editing of exon 10 replaced a lysine with glutamic acid (nucleotide sequence at aa 302: AAA to GAA) to express the Ptprca (CD45.1) rather than the Ptprcb (CD45.2) isoform normally expressed by C57BL/6J mice. Four silent mutations were introduced in order to prevent cutting of the mutated allele by the Cas9 protein. (J:358634)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ptprc Mutation: 212 strains or lines available

References

• Original: J:358634 Laubreton D, et al., Generation of a C57BL/6J mouse strain expressing the CD45.1 epitope to improve hematopoietic stem cell engraftment and adoptive cell transfer experiments. Lab Anim (NY). 2023 Dec;52(12):324-331
• All: 1 reference(s)