Tg(HBB)31invJeng
Transgene Detail

Summary

• Symbol: Tg(HBB)31invJeng
• Name: transgene insertion 31inv, James Douglas Engel
• MGI ID: MGI:7861315
• Synonyms: 31-inv.
• Transgene: Tg(HBB)31invJeng Location: unknown
• Alliance: Tg(HBB)31invJeng page

Transgene origin

• Strain of Origin: CD-1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene comprises a modified 150 kb human HBB cluster-containing YAC A201F4.3. The modification involves the insertion of a loxP site between the LCR and the HBE1 gene and a second loxP site in opposite orientation downstream of the HBB gene and the subsequent Cre-mediated inversion of the HBB cluster with respect to the upstream LCR. Southern blot analysis confirmed the integrity and single copy number of YAC insertion. Embryonic expression of HBE1, HBG1 and HBG2 is significantly reduced compared to the non-inverted transgene. (J:121690)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:121690 Tanimoto K, et al., Effects of altered gene order or orientation of the locus control region on human beta-globin gene expression in mice. Nature. 1999 Mar 25;398(6725):344-8
• All: 2 reference(s)