Tcf21^tm1Cswt
Targeted Allele Detail

Summary

• Symbol: Tcf21^tm1Cswt
• Name: transcription factor 21; targeted mutation 1, Christopher H Switzer
• MGI ID: MGI:7860772
• Synonyms: Tcf21^tm(rtTA)
• Gene: Tcf21 Location: Chr10:22693162-22696042 bp, - strand Genetic Position: Chr10, 10.33 cM
• Alliance: Tcf21^tm1Cswt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Transactivator)
• Mutation: Insertion
• Mutation details: Reverse Tet-off rtTA protein controlled by Tcf21 promoter (fibroblast-specific). A 9.1 kb region was first subcloned from a positively identified C57BL/6 fosmid clone (WI1-336E22) and used to build the targeting vector using homologous recombination-based techniques. The region is designed so that the long homology arm (LA) extends 6.2 kb upstream of the P2A-rtTA knock-in cassette and the short homology arm (SA) extends 2.3 kb downstream of the neo cassette. The P2A-rtTA cassette was inserted upstream of the TGA stop codon in exon 2 of the mouse Tcf21 gene. The FRT flanked neo selection cassette followed downstream of the 3-UTR sequence. The targeting vector was confirmed by restriction analysis and sequencing after each modification step. The boundaries of the 2 homology arms were confirmed by sequencing with primers P6 and T73 that read through both sides of the backbone vector into the genomic sequences. The P2A-rtTA knock-in cassette was confirmed by sequencing with primers TCFC SQ1 and SQ2. IV-neo N3 and ineo N2 primers, reading from the selection cassette, confirmed the insertion of the neo cassette. The PTRE3G promoter has been used. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tcf21 Mutation: 16 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)