Rps6ka1^tm1.1Peat
Targeted Allele Detail

Summary

• Symbol: Rps6ka1^tm1.1Peat
• Name: ribosomal protein S6 kinase polypeptide 1; targeted mutation 1.1, Philip Eaton
• MGI ID: MGI:7860766
• Synonyms: Cys421Ser/Cys424Ser RSK1
• Gene: Rps6ka1 Location: Chr4:133574601-133615108 bp, - strand Genetic Position: Chr4, 66.37 cM, cytoband D3
• Alliance: Rps6ka1^tm1.1Peat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Constitutively active)
• Mutation: Nucleotide substitutions
• Mutation details: Constitutive global C421S and C424S mutations knock-in in the mouse Rps6ka1 (Rsk1) gene. The Rps6ka1 gene (GenBank accession number: NM_009097.4, Ensembl: ENSMUSG00000003644) is located on mouse chromosome 4; 22 exons have been identified, with the ATG start codon in exon 1 and TGA stop codon in exon 22; the amino acid residues C421 and C424 are located in exon 15. To engineer the targeting vector, 5 homology arm and 3 homology arm were amplified from BAC DNA and confirmed by end sequencing. The C421S mutation (TGT to TCT) and C424S mutation (TGT to TCT) were introduced into exon 15 in 3 homology arm. In the targeting vector, the neo cassette was flanked by loxP sites. DTA was used for negative selection. The constitutive knock-in allele was obtained after cre-mediated recombination. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rps6ka1 Mutation: 76 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)