Gt(ROSA)26Sor^{tm14(RARE-CFP)Mgn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm14(RARE-CFP)Mgn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 14, Mark A Magnuson
• MGI ID: MGI:7857092
• Synonyms: ROSA26R26-228-DR5-TA-Cerulean
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm14(RARE-CFP)Mgn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195112
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: This allele was generated using RMCE to insert an exchange vector containing a modified Rosa26 promoter linked to a Cerulean fluorescent protein (CFP) reporter gene into mESCs containing a Loxed Cassette Acceptor (LCA) allele within the Rosa26 gene locus. The Rosa26 promoter in this mouse was altered by replacing DNA sequences from 228 to +81 with a multimerized retinoic acid response element (DR5) fused to a TATA box. This mouse will facilitate studies of retinoic acid signaling in an intact animal. In this experiment native Rosa26 gene sequence from -228 to +81 will be replaced by a retinoic acid response element (DR5) fused to a TATACerulean red reporter. The resulting mouse ESCs will enable the feasibility of inserting signaling sentinel cassettes into a facilitating chromosomal locus to be directly tested. (J:195112)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:195112 Serup P, et al., Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. Dis Model Mech. 2012 Nov;5(6):956-66
• All: 1 reference(s)