Tg(Rr596*-EGFP)nPE1mutcoreLow
Transgene Detail

Summary

• Symbol: Tg(Rr596*-EGFP)nPE1mutcoreLow
• Name: transgene insertion nPE1mutcore, Malcolm J Low
• MGI ID: MGI:7855605
• Synonyms: nPE1corePomc(mut)-EGFP
• Transgene: Tg(Rr596*-EGFP)nPE1mutcoreLow Location: unknown
• Alliance: Tg(Rr596*-EGFP)nPE1mutcoreLow page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene contains the following: the Pomc gene, including sequence 8 kb downstream of the transcription start site and 13 kb upstream (minus the -6.5 to -0.8 kb region), containing pituitary-specific enhancer Rr281 but with neuron-specific enhancer Rr280 (nPE2) deleted (172 bp) and Rr279 (nPE1) still present, and the promoter, with the EGFP reporter gene and SV40 poly(A) signal sequences inserted upstream of the translation start site. The nPE1 enhancer core sequence was modified with three homeodomain binding sites (HDBSs) mutated from TCAAG, TAAT and ATTA to TCCCG, TCCT and ACCA. (J:224010)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:224010 Lam DD, et al., Partially redundant enhancers cooperatively maintain Mammalian pomc expression above a critical functional threshold. PLoS Genet. 2015 Feb;11(2):e1004935
• All: 1 reference(s)