Zfp57^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Zfp57^{tm1b(EUCOMM)Hmgu}
• Name: zinc finger protein 57; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7840196
• Gene: Zfp57 Location: Chr17:37312055-37321527 bp, + strand Genetic Position: Chr17, 19.16 cM, cytoband B3
• Alliance: Zfp57^{tm1b(EUCOMM)Hmgu} page
• IMPC: Zfp57 gene page

Mutation origin

• Mutant Cell Line: HEPD0900_6_E12
• Germline Transmission: Earliest citation of germline transmission: J:361968
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_GT1_LF2A_LacZ_BetactP_neo
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 55945874 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 55949099. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:361968)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zfp57 Mutation: 40 strains or lines available

References

• Original: J:361968 Mary Lyon Centre at MRC Harwell, Direct data submission from Mary Lyon Centre at MRC Harwell. 2025
• All: 1 reference(s)