Polr2f^{tm1e(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Polr2f^{tm1e(EUCOMM)Wtsi}
• Name: polymerase (RNA) II (DNA directed) polypeptide F; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:7840179
• Gene: Polr2f Location: Chr15:79025525-79035974 bp, + strand Genetic Position: Chr15, 37.7 cM
• Alliance: Polr2f^{tm1e(EUCOMM)Wtsi} page
• IMPC: Polr2f gene page

Mutation origin

• Mutant Cell Line: EPD0241_4_A05
• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 79029712 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Polr2f Mutation: 24 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 2 reference(s)