Spocd1^em3Doca
Endonuclease-mediated Allele Detail

Summary

• Symbol: Spocd1^em3Doca
• Name: SPOC domain containing 1; endonuclease-mediated mutation 3, Donal O'Carroll
• MGI ID: MGI:7779621
• Synonyms: Spocd1^deltaSPIN1
• Gene: Spocd1 Location: Chr4:129823042-129850908 bp, + strand Genetic Position: Chr4, 63.43 cM
• Alliance: Spocd1^em3Doca page

Mutation origin

• Strain of Origin: (C57BL/6J x CBA/CaCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology generated a mutation of eight amino acids to alanine (GIRLVGAASS to AAAAAAAAA) in exon 4 in the predicted beta-hairpin that mediates SPIN1 binding. This generates a separation-of-function allele that encodes a SPOCD1 variant that no longer interacts with SPIN1. (J:355681)

Expression

In Mice Carrying this Mutation:

7 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spocd1 Mutation: 30 strains or lines available

References

• Original: J:355681 Dias Mirandela M, et al., Two-factor authentication underpins the precision of the piRNA pathway. Nature. 2024 Oct;634(8035):979-985
• All: 1 reference(s)