Kat6b^tm2.1Avo
Targeted Allele Detail

Summary

• Symbol: Kat6b^tm2.1Avo
• Name: K(lysine) acetyltransferase 6B; targeted mutation 2.1, Anne K Voss
• MGI ID: MGI:7768130
• Gene: Kat6b Location: Chr14:21549284-21722546 bp, + strand Genetic Position: Chr14, 11.73 cM
• Alliance: Kat6b^tm2.1Avo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:348717
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6 x 129S/SvEv)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The Kat6b locus was targeted twice to generate this allele. The first targeting vector was designed to flank flanking exon 2, containing the ATG site, with loxP sites. Resulting mice containing the floxed allele were containing Kat6b^tm1Avo allele was used to generate the ES cells. The second target is to flank Kat6b exons 11-12 with loxP sites in the Kat6b. An FRT- and loxP-flanked neomycin resistance cassette was also inserted. Cre recombination deleted exons 2-12, removed all splice variants, and removed the neo cassette. (J:101977, J:348717)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kat6b Mutation: 109 strains or lines available

Notes

ES cells on a C57BL/6 x 129Sv/J F1 hybrid background were generated from mice with Kat6b^tm1Avo allele crossed to 129Sv/J mice.

References

• Original: J:348717 Bergamasco MI, et al., The histone acetyltransferase KAT6B is required for hematopoietic stem cell development and function. Stem Cell Reports. 2024 Apr 9;19(4):469-485
• All: 4 reference(s)