Flt3^{tm1.1(FLT3*)Rlvn}
Targeted Allele Detail

Summary

• Symbol: Flt3^{tm1.1(FLT3*)Rlvn}
• Name: FMS-like tyrosine kinase 3; targeted mutation 1.1, Ross Levine
• MGI ID: MGI:7768128
• Gene: Flt3 Location: Chr5:147267551-147337299 bp, - strand Genetic Position: Chr5, 86.88 cM, cytoband G3
• Alliance: Flt3^{tm1.1(FLT3*)Rlvn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: An FRT site (inverted) was placed in intron 12, and a lox2272-flanked neomycin cassette followed by an inversion cassette was introduced downstream of 15 of the mouse Flt3 gene by homologous recombination in C57BL/6-derived embryonic stem cells. The inversion cassette, carrying exons 13, 14*, and 15 (in reverse orientation) followed by an FRT site, is flanked by two divergent, outward-facing F3 sites. Exon 14* of the inversion cassette is modified to express humanized FLT3-ITD W51 (a duplication of amino acids 596 to 602. Flanking genomic sequences from upstream of exon 13 to downstream of exon 15 surround the inversion cassette for correct splicing (Inv.saE13-14*-E15Sd). The neomycin cassette was excised by transient expression of cre recombinase in the targeted ES cells. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Flt3 Mutation: 91 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)