Igs84^em1Pero
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igs84^em1Pero
• Name: intergenic site 84; endonuclease-mediated mutation 1, Pedro P Rocha
• MGI ID: MGI:7718676
• Synonyms: CTCFi3x^-
• Gene: Igs84 Location: Chr3:34748993-34748994 bp Genetic Position: Chr3, Syntenic
• Alliance: Igs84^em1Pero page

Mutation origin

• Strain of Origin: C57BL/6NCr

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Insertion
• Mutation details: The following 321 bp concatenation of three CTCF-binding-motif-containing sequences were inserted (in this order) between GRCm39:chr3:34748993 and 34748994 using an sgRNA (equivalent to CCTATCATCCTTATTCAGCTCAT) and an ssODN template using CRISPR/Cas9 technology: GRCm39:chr4:132978080-132978190, reverse complement of chr1:34257543-34257652 and chr8:13511990-13512089. (J:342647)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs84 Mutation: 0 strains or lines available

References

• Original: J:342647 Chakraborty S, et al., Enhancer-promoter interactions can bypass CTCF-mediated boundaries and contribute to phenotypic robustness. Nat Genet. 2023 Feb;55(2):280-290
• All: 1 reference(s)