Dmd^em1Kxi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dmd^em1Kxi
• Name: dystrophin, muscular dystrophy; endonuclease-mediated mutation 1, Kyoko Itoh
• MGI ID: MGI:7716702
• Gene: Dmd Location: ChrX:81992476-84249747 bp, + strand Genetic Position: ChrX, 38.38 cM, cytoband C
• Alliance: Dmd^em1Kxi page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details:

  CRISPR/Cas9 technology inserted an HA-tag at the Dp71-specific translation initiation site which is present in intron 62 to fuse with the N-terminal amino acid sequence of Dp71 in-frame. This tag allows for the specific detection of the short dystrophin product, Dp71, expression. (J:352582)

Expression

In Mice Carrying this Mutation:

201 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dmd Mutation: 162 strains or lines available

References

• Original: J:352582 Fujimoto T, et al., Generation of dystrophin short product-specific tag-insertion mouse: distinct Dp71 glycoprotein complexes at inhibitory postsynapse and glia limitans. Cell Mol Life Sci. 2022 Jan 30;79(2):109
• All: 2 reference(s)