Rbp3^em1Jmni
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rbp3^em1Jmni
• Name: retinol binding protein 3, interstitial; endonuclease-mediated mutation 1, John M Nickerson
• MGI ID: MGI:7710916
• Synonyms: IRBP^fl
• Gene: Rbp3 Location: Chr14:33675960-33686173 bp, + strand Genetic Position: Chr14, 20.8 cM
• Alliance: Rbp3^em1Jmni page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: Exon 1 of the Rbp3 gene was flanked by loxP sites using a combination of targeting in C57BL/6J derived ES cells and subsequent CRISPR/cas9 methodologies to introduce additional loxP sites. The allele Rbp3^tm1.1Jmni created a 3' loxP site at 3117 bp downstream from the transcription start site in the first intron. Then CRISPR/cas9 methodologies were used to introduce a second 5' loxP site upstream of exon 1 at -643 bp upstream from the transcription start site in heterozygous zygotes derived from IVF of wildtype C57BL/6J eggs and sperm having a single 3' loxP in the Rbp3 gene Rbp3^tm1.1Jmni. (J:353299)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rbp3 Mutation: 90 strains or lines available

References

• Original: J:353299 Getz TE, et al., Conditional Knockouts of Interphotoreceptor Retinoid Binding Protein Suggest Two Independent Mechanisms for Retinal Degeneration and Myopia. Invest Ophthalmol Vis Sci. 2024 Jun 3;65(6):32
• All: 1 reference(s)