Haao^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Haao^{tm1b(KOMP)Wtsi}
• Name: 3-hydroxyanthranilate 3,4-dioxygenase; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7705379
• Gene: Haao Location: Chr17:84138585-84155392 bp, - strand Genetic Position: Chr17, 54.85 cM, cytoband E4
• Alliance: Haao^{tm1b(KOMP)Wtsi} page
• IMPC: Haao gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:352181
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 84141911 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 84143483. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:352181)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Haao Mutation: 20 strains or lines available

References

• Original: J:352181 Dang H, et al., On the benefits of the tryptophan metabolite 3-hydroxyanthranilic acid in Caenorhabditis elegans and mouse aging. Nat Commun. 2023 Dec 14;14(1):8338
• All: 1 reference(s)