Slc44a4^tm1.2Said
Targeted Allele Detail

Summary

• Symbol: Slc44a4^tm1.2Said
• Name: solute carrier family 44, member 4; targeted mutation 1.2, Hamid M Said
• MGI ID: MGI:7703492
• Gene: Slc44a4 Location: Chr17:35133442-35149412 bp, + strand Genetic Position: Chr17, 18.46 cM, cytoband B2
• Alliance: Slc44a4^tm1.2Said page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:351314
• Parent Cell Line: iTL BF1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted 5' of exon 8 and an FRT-flanked neo cassette and loxP site were inserted 3' of exon 11 via homologous recombination. Recombinase-mediated recombination removed the neo cassette and exons 8-11. RT-qPCR and western blot analysis confirmed the absence of expression in the colon from homozygous mice. (J:351314)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc44a4 Mutation: 48 strains or lines available

References

• Original: J:351314 Sabui S, et al., Effect of knocking out mouse Slc44a4 on colonic uptake of the microbiota-generated thiamine pyrophosphate and colon physiology. Am J Physiol Gastrointest Liver Physiol. 2024 Jul 1;327(1):G36-G46
• All: 1 reference(s)