Fgf8^em2Zyliu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fgf8^em2Zyliu
• Name: fibroblast growth factor 8; endonuclease-mediated mutation 2, Zhiyong Liu
• MGI ID: MGI:7703102
• Synonyms: Fgf8^GFP, Fgf8^P2A-3xGFP
• Gene: Fgf8 Location: Chr19:45725237-45731354 bp, - strand Genetic Position: Chr19, 38.75 cM, cytoband C3-D
• Alliance: Fgf8^em2Zyliu page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9 technology, a P2A self-cleaving peptide that mediates ribosomal skipping and a 3xGFP sequence (including three distinct GFPs: Emerald-GFP (EmGFP), TagGFP2, and humanized Renilla-GFP (hrGFP)) were inserted immediately upstream of the endogenous stop codon of the fibroblast growth factor 8 (Fgf8) gene. (J:351381)

Expression

In Mice Carrying this Mutation:

27 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fgf8 Mutation: 25 strains or lines available

References

• Original: J:351381 Pan Y, et al., Fgf8(P2A-3xGFP/+): A New Genetic Mouse Model for Specifically Labeling and Sorting Cochlear Inner Hair Cells. Neurosci Bull. 2023 Dec;39(12):1762-1774
• All: 2 reference(s)