Gt(ROSA)26Sor^{em1.1(CAG-cas12a*)Sidi}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1.1(CAG-cas12a*)Sidi}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, Sidi Chen
• MGI ID: MGI:7662592
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1.1(CAG-cas12a*)Sidi} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Endonuclease)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em1.1(CAG-cas12a*)Sidi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: Using CRISPR/Cas9 technology, the intron 1 of the mouse Gt(ROSA)26Sor locus was targeted to insert LoxP-Stop-LoxP (LSL)-codon-optimized enAsCas12a-HF1 cDNA. EnAsCas12a-HF1 is an engineered, high-fidelity version of the Acidaminococcus sp. Cas12a variant (AsCas12a) with substitutions E174R (GAG to AGA)/N282A (AAT to GCC)/S542R (TCT to AGA)/K548R (AAG to AGA), an expanded PAM sequence, and enhanced multiplexed gene editing efficiency. Egl-13, a nuclear localizing signal (NLS) derived from C. elegans, was placed at the N-terminus, and the nucleoplasmin NLS and c-Myc NLS signals were placed on the C-terminus of enAsCas12a-HF1 to maintain a higher concentration of enAsCas12a-HF1 in the nucleus and thus enhance gene editing efficiency. The cre-recombinase removed excise the loxP-flanked Stop. (J:101977, J:363522)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1042 strains or lines available

References

• Original: J:363522 Tang K, et al., Cas12a-knock-in mice for multiplexed genome editing, disease modelling and immune-cell engineering. Nat Biomed Eng. 2025 Mar 20
• All: 2 reference(s)