Cep192^{em1(mNeonGreen*)Ahol}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cep192^{em1(mNeonGreen*)Ahol}
• Name: centrosomal protein 192; endonuclease-mediated mutation 1, Andrew Holland
• MGI ID: MGI:7657344
• Synonyms: Cep192^{mAID-mNeonGreen}
• Gene: Cep192 Location: Chr18:67933177-68018241 bp, + strand Genetic Position: Chr18, 40.11 cM, cytoband E1
• Alliance: Cep192^{em1(mNeonGreen*)Ahol} page

Mutation origin

• Strain of Origin: (C57BL/6 x SJL)F2

Mutation description

• Allele Type: Endonuclease-mediated (Inducible degradation, Reporter)
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9 technology, a single guide RNA [5'-cgactaatcggtgaatccct-3'] was used to introduce a flexible linker, mAID [miniaturized auxin-inducible degradation-promoting sequence (degron) tag] and moxNeonGreen sequence into exon 48 of the centrosomal protein 192 (Cep192) gene on chromosome 18. (J:364098)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cep192 Mutation: 75 strains or lines available

References

• Original: J:364098 Sladky VC, et al., Rapid and sustained degradation of the essential centrosome protein CEP192 in live mice using the AID2 system. Sci Adv. 2025 Feb 28;11(9):eadq2339
• All: 1 reference(s)