Il6^em1(IL6)Akp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Il6^em1(IL6)Akp
• Name: interleukin 6; endonuclease-mediated mutation 1, Karolina Palucka
• MGI ID: MGI:7628228
• Synonyms: Il6^emX(IL6)Akp
• Gene: Il6 Location: Chr5:30218112-30224973 bp, + strand Genetic Position: Chr5, 15.7 cM
• Alliance: Il6^em1(IL6)Akp page

Mutation origin

• Strain of Origin: NOD.Cg-Flt3^em1Akp Prkdc^scid Il2rg^tm1Wjl/J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Il6^em1(IL6)Akp expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  IL6 (3569)

  Il6	(MGI:96559)

  replaces mouse Il6

• Mutation details: Using a CRISPR/Cas9 system, Cas9 mRNA, sgRNAs targeting mouse Il6 and recombinant human IL6 genomic DNA were co-injected into fertilized NOD.Cg-Flt3^em1Akp Prkdc^scid Il2rg^tm1Wjl/J oocyte. Full-length human IL6 sequence replaces exons 1-5 of the mouse Il6 gene. (J:347776)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il6 Mutation: 39 strains or lines available

References

• Original: J:347776 Yu CI, et al., Engraftment of adult hematopoietic stem and progenitor cells in a novel model of humanized mice. iScience. 2024 Mar 15;27(3):109238
• All: 1 reference(s)