Gt(ROSA)26Sor^{em117(CAG-Gpld1,-tdTomato)Smoc}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em117(CAG-Gpld1,-tdTomato)Smoc}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 117, Shanghai Model Organisms Center
• MGI ID: MGI:7623919
• Synonyms: GPLD1-Tg, Gt(ROSA)26Sor^{em117(CAG-Gpld1,-dTomato}, Gt(ROSA)26Sor^{em1(CAG-LSL-Gpld1-IRES-tdTomato-Wpre-pA)Smoc}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em117(CAG-Gpld1,-tdTomato)Smoc} page

Mutation origin

• Strain of Origin: C57BL/6JSmoc

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em117(CAG-Gpld1,-tdTomato)Smoc} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gpld1 (MGI:106604)

• Gt(ROSA)26Sor^{em117(CAG-Gpld1,-tdTomato)Smoc} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: CRISPR-targeting introduced the mouse gene and reporter gene. A CAG-LSL-Gpld1-IRES-tdTomato-Wpre-pA expression cassette was inserted into the Rosa26 locus via homologous recombination. The construct contained the full-length mouse Gpld1 cDNA under the control of a CAG promoter, followed by a loxP-stop-loxP sequence, IRES-tdTomato reporter, and WPRE-pA terminator. (J:238766)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1214 strains or lines available

References

• Original: J:238766 Shanghai Model Organisms Center, Information obtained from the Shanghai Model Organisms Center (SMOC), Shanghai, China. MGI Direct Data Submission. 2017
• All: 2 reference(s)