Idh2^tm1Makt
Targeted Allele Detail

Summary

• Symbol: Idh2^tm1Makt
• Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial; targeted mutation 1, Tak Mak
• MGI ID: MGI:7618353
• Synonyms: Idh2^LSL-R140Q, IDH2R140Q
• Gene: Idh2 Location: Chr7:79744594-79765140 bp, - strand Genetic Position: Chr7, 45.43 cM
• Alliance: Idh2^tm1Makt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239062
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Null/knockout)
• Mutations: Insertion, Single point mutation
• Mutation details: Arginine codon 140 (CGG) in exon 4 was changed to glutamine (CAG) (p.R140Q) and a loxP site flanked STOP cassette (puromycin resistance gene, splice acceptor sequence and four copies of SV40 poly(A) signal sequence) was inserted into intron 3. This is a knockout allele and only after Cre-mediated removal of the STOP cassette will it express the mutated transcript and peptide. (J:239062)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Idh2 Mutation: 145 strains or lines available

References

• Original: J:239062 Lemonnier F, et al., The IDH2 R172K mutation associated with angioimmunoblastic T-cell lymphoma produces 2HG in T cells and impacts lymphoid development. Proc Natl Acad Sci U S A. 2016 Dec 27;113(52):15084-15089
• All: 2 reference(s)