Gt(ROSA)26Sor^{tm1.2(CAG-rtTA3,-GFP*,-luc*)Zhrt}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.2(CAG-rtTA3,-GFP*,-luc*)Zhrt}
• Name: gene trap ROSA 26, Philippe Soriano; transgene insertion 1.2, Zachary Hartman
• MGI ID: MGI:7611706
• Synonyms: NoGlow+
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.2(CAG-rtTA3,-GFP*,-luc*)Zhrt} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: The targeting vector was designed with (from 5' to 3') a ubiquitously expressed CAG promoter, a loxP-flanked stop cassette, a reverse tetracycline-regulated transactivator (rtTA3) sequence, P2A self-cleaving sequence that mediates ribosomal skipping, a mutant form of GFP, a T2A self-cleaving sequence, and a mutant form of Luciferase (luc). A frt-flanked blue fluorescence protein (eBFP2) sequence was inserted downstream. Also, a neomycin resistance (neo) cassette is present in the floxed region. This targeting vector was inserted into the Gt(ROSA)26Sor locus. Specifically, the targeting vector contains antigen-intact but non-functional forms of GFP (Delta T64) and luc (G315A). FLP recombinase removed eBFP2 cassette. Cre-recombinase removed the loxP-flanked stop cassette. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)