Tg(Gtf2ird1)KrnJvvw
Transgene Detail

Summary

• Symbol: Tg(Gtf2ird1)KrnJvvw
• Name: transgene insertion Krn, Jeremy Veenstra-VanderWeele
• MGI ID: MGI:7607006
• Transgene: Tg(Gtf2ird1)KrnJvvw Location: unknown
• Alliance: Tg(Gtf2ird1)KrnJvvw page

Transgene origin

• Strain of Origin: C57BL/6NTac

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Gtf2ird1)KrnJvvw expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gtf2ird1 (MGI:1861942)

• Mutation details: A bacterial artificial chromosome which contained the entirety of the 100 kb Gtf2ird1 gene, and 89 kb of flanking regulatory sequence (60 kb at the 5 end and 28 kb at the 3), but no additional intact genes or their promoters was utilized to generate the transgene. This was then recombined using standard methods to insert an HA tag in-frame directly before the stop codon of the beta isoforms 208,209. Homologous recombination via transient expression of RecA was employed, followed by Neomycin selection of an inserted FRT-flanked cassette. The selection cassette was then removed after the expression of FLPe, leaving a single FRT site downstream of the stop codon. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)