Gt(ROSA)26Sor^{tm3(CAG-mCherry,-EGFP*)Rray}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-mCherry,-EGFP*)Rray}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Russell Ray
• MGI ID: MGI:7587845
• Synonyms: RC::FP_L10A-EGFP, RR3
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-mCherry,-EGFP*)Rray} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: This allele is responsive to Cre and FLP recombinases. Upon FLP recombination, cells will express mCherry. Upon Cre/FLP dual recombination, cells will express L10A-EGFP. It can be used to delineate cell types by dual expression of Cre and FLP recombinases through transgenic transmission or virus. Upon dual recombination, intersectional targeted cells will express L10A-EGFP, an L10A ribosomal subunit fused to EGFP that can be used for RiboTRAP assays for pull-down of actively translating mRNA. It is useful in studies on the role of mRNA expression in cells defined by Cre/FLP in a variety of behavioural and physiological assays with high spatial and temporal resolution. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1096 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 2 reference(s)