Gt(ROSA)26Sor^{tm1(CAG-mCherry/CHRM3*)Rray}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-mCherry/CHRM3*)Rray}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Russell Ray
• MGI ID: MGI:7587843
• Synonyms: RC::FP_hM3D, RR2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-mCherry/CHRM3*)Rray} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: This allele is responsive to Cre and FLP recombinases. Upon FLP recombination, cells will express mCherry. Upon Cre/FLP dual recombination, cells will express hM3D. It can be used to delineate cell types by dual expression of Cre and FLP recombinases through transgenic transmission or virus. Upon dual recombination, intersectional targeted cells will express hM3D, a modified hM3 receptor that can be activated using Clozapine-N-Oxide (CNO), a synthetic drug, for Gs-mediated perturbation of cells. It is useful in studies on the role of any cells defined by Cre/FLP in a variety of behavioral and physiological assays with high spatial and temporal resolution. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 3 reference(s)