Gt(ROSA)26Sor^{em2(CAG-mCherry,-EGFP*)Rray}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em2(CAG-mCherry,-EGFP*)Rray}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, Russell Ray
• MGI ID: MGI:7587831
• Synonyms: RC::FP_Rs-eGFP, RR7
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em2(CAG-mCherry,-EGFP*)Rray} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: This allele is responsive to Cre and FLP recombinases. Upon FLP recombination, cells will express mCherry. Upon Cre/FLP dual recombination, cells will express the Rs (M3 receptor Gs RASSL) fused to eGFP. It can be used to delineate cell types by dual expression of Cre and FLP recombinases through transgenic transmission or virus. Upon dual recombination, intersectional targeted cells will express the Rs RASSL, allowing for Gs-mediated perturbation of cells upon Clozapine-N-oxide (CNO). This is useful in studies on the role of any cells defined by Cre/FLP in a variety of behavioral and physiological assays with high spatial and temporal resolution. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 2 reference(s)