Nfkbid^em3Dvs
Endonuclease-mediated Allele Detail

Summary

• Symbol: Nfkbid^em3Dvs
• Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, delta; endonuclease-mediated mutation 3, David V Serreze
• MGI ID: MGI:7580893
• Gene: Nfkbid Location: Chr7:30121157-30128171 bp, + strand Genetic Position: Chr7, 17.52 cM
• Alliance: Nfkbid^em3Dvs page

Mutation origin

• Strain of Origin: NOD

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/cas9 mediated recombination using a single guide RNA (5'-AGGCCCATTTCCCCTGGTGA-3') created an 8-nt deletion and an inverted insertion of 154 nucleotides copied from exon 2 removing the translation start site in exon 3. Western blotting of thymus from homozygotes found no NFKBID protein. (J:265223)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfkbid Mutation: 20 strains or lines available

References

• Original: J:265223 Presa M, et al., A Hypermorphic Nfkbid Allele Contributes to Impaired Thymic Deletion of Autoreactive Diabetogenic CD8(+) T Cells in NOD Mice. J Immunol. 2018 Oct 1;201(7):1907-1917
• All: 3 reference(s)