Gt(ROSA)26Sor^{tm1.1(CAG-CCre,-NCre)Syao}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-CCre,-NCre)Syao}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Shenqin Yao
• MGI ID: MGI:7573052
• Synonyms: RCL-CreV
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-CCre,-NCre)Syao} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Recombinase)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(CAG-CCre,-NCre)Syao} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The targeting vector is designed with (from 5' to 3') a ubiquitously expressed CAG (CMV enhancer/chicken beta-actin) promoter, a floxed STOP cassette (containing polyadenylation [pA] sites), CreV, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA, an attB site, a PGK-Neo cassette with a PGK poly(A), and an attP site. CreV consists of a split, light-inducible Cre, with the N-terminal CreV (NCreV) and C-terminal CreV (CCreV) protein sequences separated by an internal ribosome entry site. PhiC31o-mediated recombination removed the neo cassette. (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1058 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)