Gt(ROSA)26Sor^{tm1.1(CAG-Ormdl3)Sspi}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-Ormdl3)Sspi}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Sarah Spiegel
• MGI ID: MGI:7572532
• Synonyms: Ormdl3-Flag
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-Ormdl3)Sspi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341336
• Parent Cell Line: BAC-BA1 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S/SvEv)F1

Mutation description

• Allele Type: Targeted (Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(CAG-Ormdl3)Sspi} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ormdl3 (MGI:1913862)

• Mutation details: The targeting construct contains (from 5' to 3'): a CAG promoter, loxP-STOP-loxP (LSL), a 3X FLAG epitope, Ormdl3 cDNA, polyA sequence flanked by ROSA26 5' and 3' homology arms and inserted into the Gt(ROSA)26Sor locus. Cre-mediated recombination removed the LSL sequence. (J:341336)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:341336 James BN, et al., Neutrophilia in severe asthma is reduced in Ormdl3 overexpressing mice. FASEB J. 2023 Mar;37(3):e22799
• All: 1 reference(s)