Smg6^tm1.1Tac
Targeted Allele Detail

Summary

• Symbol: Smg6^tm1.1Tac
• Name: SMG6 nonsense mediated mRNA decay factor; targeted mutation 1.1, Taconic
• MGI ID: MGI:7571582
• Synonyms: Smg6^flox, Smg6^{tm5498(D1352A,D1391A)Tac}
• Gene: Smg6 Location: Chr11:74816665-75055274 bp, + strand Genetic Position: Chr11, 45.76 cM
• Alliance: Smg6^tm1.1Tac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342742
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site, an FRT site flanked neomycin resistance gene cassette, cDNA sequence for exons 17-19, human GH gene poly(A) signal sequences, an F3 site flanked puromycin resistance gene cassette and a second loxP site were inserted into intron 16. Aspartic acid codons 1352 (GAT) and 1391 (GAT) in endogenous exons 18 and 19 were changed to alanine (GCC) (p.D1352A, p.D1391A). These mutations, in the PIN (PilT N terminus) nuclease domain of the encoded peptide, render the protein NMD-inactive. The neo and puro cassettes were removed through subsequent Flp-mediated recombination. This allele expresses wildtype peptides and only after Cre-mediated deletion of the exon 17-19 and pol(A) signal sequences will it express the mutated peptides. (J:342742)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smg6 Mutation: 98 strains or lines available

References

• Original: J:342742 Katsioudi G, et al., A conditional Smg6 mutant mouse model reveals circadian clock regulation through the nonsense-mediated mRNA decay pathway. Sci Adv. 2023 Jan 13;9(2):eade2828
• All: 1 reference(s)