Taf1^{em1(EEF1A1-mNeonGreen,TAF1*)Jboe}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Taf1^{em1(EEF1A1-mNeonGreen,TAF1*)Jboe}
• Name: TATA-box binding protein associated factor 1; endonuclease-mediated mutation 1, Jef Boeke
• MGI ID: MGI:7565694
• Synonyms: Taf1^PC-XDP
• Gene: Taf1 Location: ChrX:100576335-100644635 bp, + strand Genetic Position: ChrX, 44.29 cM
• Alliance: Taf1^{em1(EEF1A1-mNeonGreen,TAF1*)Jboe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9-techology, an initial loxP site was inserted into intron 24 of the endogenous TATA-box binding protein associated factor 1 (Taf1) locus. After which a marker cassette, and a second loxP site, are followed by an 81-kb fragment of a patient-derived human TAF1 genomic sequence from intron 24 through the 3' UTR, including the XDP-associated SVA_F retrotransposon containing 34 contiguous hexanucleotide repeats, were introduced via homologous recombination. The marker cassette includes a human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) promoter, mNeonGreen sequence, a human mini hypoxanthine phosphoribosyltransferase 1 (HPRT1) gene, T2A self-cleaving peptide sequence, a neomycin resistance gene, and a Simian virus 40 PolyA (SV40pA) sequence. There is also a STOP codon in the marker cassette (in NeoR), as well as the native STOP codon in human TAF1 exon 38. TAF1 human coding exons 25-38 have been engineered as needed to code for a fully mouse TAF1 protein. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Taf1 Mutation: 13 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)