Fbxl12^tm1Lov
Targeted Allele Detail

Summary

• Symbol: Fbxl12^tm1Lov
• Name: F-box and leucine-rich repeat protein 12; targeted mutation 1, Paul E Love
• MGI ID: MGI:7565495
• Gene: Fbxl12 Location: Chr9:20549045-20556064 bp, - strand Genetic Position: Chr9, 7.59 cM, cytoband A3
• Alliance: Fbxl12^tm1Lov page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280728
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector obtained from KOMP is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:280728)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fbxl12 Mutation: 30 strains or lines available

References

• Original: J:280728 Zhao B, et al., Notch and the pre-TCR coordinate thymocyte proliferation by induction of the SCF subunits Fbxl1 and Fbxl12. Nat Immunol. 2019 Oct;20(10):1381-1392
• All: 1 reference(s)