Gzmb^em1Bros
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gzmb^em1Bros
• Name: granzyme B; endonuclease-mediated mutation 1, Nils Brose
• MGI ID: MGI:7565277
• Synonyms: Gzmb^em1Jret, GzmB-mTFP-KI
• Gene: Gzmb Location: Chr14:56496295-56499717 bp, - strand Genetic Position: Chr14, 28.19 cM, cytoband D3
• Alliance: Gzmb^em1Bros page

Mutation origin

• Strain of Origin: C57BL/6JRj

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 targeting replace the endogenous Stop codon with sequences encoding a GGSGGSGGS-linker, mTFP reporter gene, and a Stop codon. (J:293993)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gzmb Mutation: 35 strains or lines available

References

• Original: J:293993 Chitirala P, et al., Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse. Elife. 2020 Jul 22;9:e58065
• All: 2 reference(s)