Prom1^{tm1.1(cre/ERT2)Cle}
Targeted Allele Detail

Summary

• Symbol: Prom1^{tm1.1(cre/ERT2)Cle}
• Name: prominin 1; targeted mutation 1.1, Hans Clevers
• MGI ID: MGI:7564263
• Synonyms: Prom1^{-mCherry-(IRES)-CreERT2}
• Gene: Prom1 Location: Chr5:44150962-44259374 bp, - strand Genetic Position: Chr5, 23.97 cM
• Alliance: Prom1^{tm1.1(cre/ERT2)Cle} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:343157
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Prom1^{tm1.1(cre/ERT2)Cle} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prom1 (MGI:1100886)

• Mutation details: The targeting construct inserted the -mCherry-(IRES)-CreERT2 followed by loxP flanked neo cassette into the exon 27 of the Prom1 gene just in front of the stop codon. The neomycin selection cassette was excised in vivo by crossing to cre-deleter. (J:343157)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prom1 (mouse) Summary of all recombinase alleles driven by Prom1. Comparative matrix view of recombinase activities Alliance mouse Prom1 gene page

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prom1 Mutation: 75 strains or lines available

References

• Original: J:343157 Snippert HJ, et al., Prominin-1/CD133 marks stem cells and early progenitors in mouse small intestine. Gastroenterology. 2009 Jun;136(7):2187-2194.e1
• All: 1 reference(s)