Gt(ROSA)26Sor^{em1(CAG-Mapk1*/mTagBFP2,-H2B/iRFP713,-NFATC2*/mRuby3)Hyku}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-Mapk1*/mTagBFP2,-H2B/iRFP713,-NFATC2*/mRuby3)Hyku}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Hao Kueh
• MGI ID: MGI:7562151
• Synonyms: DPR, Rosa26^LSL-DPR
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-Mapk1*/mTagBFP2,-H2B/iRFP713,-NFATC2*/mRuby3)Hyku} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 genome editing technology is used to insert a construct containing from 5’ to 3’: a CAG promoter, a loxP-neomycin cassette-loxP (LSL) cassette followed by an Erk (formally, Mapk1) kinase translocation reporter (KTR) fused to the mTagBFP2 reporter, a tP2AT2A sequence, a human histone 2B chromatin/nucleus tag fused to the iRFP713 reporter, an IRES, and the N terminal regulatory domain (residues 1-399) of mouse Nfatc2 fused to the mRuby3 reporter inserted after exon 1 of the Gt(ROSA)26Sor locus. The ERK-KTR sensor consists of an ERK1/2 binding site and phosphorylation motif adjacent to phosphorylation activated nuclear export signal (NES) and nuclear localization sequence (NLS) fused to a fluorescent protein (mTagBFP2). (J:342890, J:349935)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1084 strains or lines available

References

• Original: J:342890 Wither MJ, et al., Antigen perception in T cells by long-term Erk and NFAT signaling dynamics. bioRxiv. 2023
• All: 2 reference(s)