Hprt1^{em2(CAG-cas9,-EGFP)Jtu}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hprt1^{em2(CAG-cas9,-EGFP)Jtu}
• Name: hypoxanthine phosphoribosyltransferase 1; endonuclease-mediated mutation 2, James Turner
• MGI ID: MGI:7561603
• Synonyms: Hprt1^{tm4(CAG-cas9,-EGFP)Jtu}, Hprt^{em2(cas9-eGFP)Jtu}, X^Cas9
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{em2(CAG-cas9,-EGFP)Jtu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:316814
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Endonuclease, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: CRISPR-targeting introduced a construct containing a CAG promoter, triple FLAG tag, nuclear localization sequence, cas9, T2A, EGFP reporter gene, and floxed neomycin resistance cassette into the locus. (J:316814)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1280 strains or lines available

References

• Original: J:316814 Douglas C, et al., CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes. Nat Commun. 2021 Dec 3;12(1):6926
• All: 1 reference(s)