Uty^{tm1(CAG-cas9,-eGFP)Jtu}
Targeted Allele Detail

Summary

• Symbol: Uty^{tm1(CAG-cas9,-eGFP)Jtu}
• Name: ubiquitously transcribed tetratricopeptide repeat containing, Y-linked; targeted mutation 1, James Turner
• MGI ID: MGI:7561601
• Synonyms: Y^Cas9
• Gene: Uty Location: ChrY:1096861-1245759 bp, - strand Genetic Position: ChrY, Syntenic
• Alliance: Uty^{tm1(CAG-cas9,-eGFP)Jtu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:316814
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced a construct containing a triple FLAG tag, nuclear localization sequence, cas9, T2A, EGFP reporter gene and floxed neomycin resistance cassette at the end of exon 30. (J:316814)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Uty Mutation: 66 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:316814 Douglas C, et al., CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes. Nat Commun. 2021 Dec 3;12(1):6926
• All: 1 reference(s)