Clec9a^tm6Crs
Targeted Allele Detail

Summary

• Symbol: Clec9a^tm6Crs
• Name: C-type lectin domain family 9, member a; targeted mutation 6, Caetano Reis e Sousa
• MGI ID: MGI:7560647
• Synonyms: Clec9a^{tm6(W155A W250A and p2A_zsGreen)Crs}
• Gene: Clec9a Location: Chr6:129385825-129401726 bp, + strand Genetic Position: Chr6, 63.3 cM
• Alliance: Clec9a^tm6Crs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342817
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting construct introduced the mutations W155A in exon 5 and W250A in exon 7 and a P2A_zsGreen immediately prior to the STOP codon. Both W to A mutations were made by mutating a Trp codon TGG to the Ala codon GCC. Exons 5, 6 and 7 and the P2A-zsGreen gene are also all flanked by a pair of loxP sites, thus allowing for the generation of a global or cell type specific knockout of the mutant protein following appropriate Cre expression. (J:342817)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clec9a Mutation: 29 strains or lines available

References

• Original: J:342817 Reis E Sousa C, SYK ubiquitination by CBL E3 ligases restrains cross-presentation of dead cell-associated antigens by type 1 dendritic cells. MGI Direct Data Submission. 2023
• All: 1 reference(s)